Spc of Salicylated

In: Other Topics

Submitted By aadesanya3
Words 321
Pages 2
THE PASSAGE OF SALYCILATE INTO YEAST CELLS

AIM: Aim of the experiment is to investigate and analyse the effect of pH on the transfer of salicylate across membranes.
INTRODUCTION: As Schedule
METHOD: As Schedule

RESULTS:

Test Tube number | Absorbance | 1 | 0 | 2 | 0.171 | 3 | 0.337 | 4 | 0.496 | 5 | 0.598 | 6 | 0.801 | 7 | 1.598 | 8a | 0.46 | 9a | 0.629 | 10a | 0.701 | 11a | 0.899 | 8b | 0.704 | 9b | 0.793 | 10b | 0.830 | 11b | 0.877 |

1. 1.0 x (PCV/100)X0.9= Volume of yeast
The PCV= 18%
:. 1.0x (18/100)x 0.9 = VY
Vy= 0.162 2. Total volume- yeast volume= volume of supernatant
:. 3.0ml- 0.162 = 2.838

3. Concentration of salicylate | Sal.concentration | V.S x C.S | Amount of sal. In yeast cells | Conc of sal. Inside yeast | 1 | 0 | 0 | 0 | 0 | 2 | 0.1 | 0.2838 | 1.7162 | 10.59 | 3 | 0.2 | 0.5876 | 1.4324 | 8.84 | 4 | 0.3 | 0.8514 | 1.1486 | 7.09 | 4 | 0.4 | 1.1352 | 0.8648 | 5.34 | 6 | 0.5 | 1.419 | 0.581 | 3.59 | 7 | 1.0 | 2.838 | -0.838 | 5.17 |

4. Volume of supernatant x concentration of salicylate
:.
0 x2.838 = 0 0.1 x 0.2838 = 0.2838 0.2 x 0.2383 =0.5876 0.3 x 0.2383 = 0.8514 0.4 x 0.2383 = 1.1352 0.5 x 0.2383 = 1.419 0.6 x 0.2383 = 2.838 5. Amount of salicylate in yeast cells= 2mg-amount of salicylate in supernatant
2mg.
( Please refer to table 2)

6. Concentration of salicylate inside yeast = amount of sal./ V.Y
(Please refer to table 2).

7. PH = PKa + log(salt/acid) = pKa+ log{A-/AH}

:.
2.9 =3.0 + log{A-/AH} PkA 3.0 for salicylate
2.9 – 3.0…...

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...Additional Tailored Allowance (W/sf) 3,4 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Tailored Ventilation (cfm/sf) 5 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Process Loads (W/sf) 5 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.00 0.00 Space Name EL1 South Perim Spc (G.S1) EL1 East Perim Spc (G.E2) EL1 NNE Peri...Spc (G.NNE3) EL1 West Perim Spc (G.W4) EL1 North Perim Spc (G.N5) EL1 North Perim Spc (G.N6) EL1 East Perim Spc (G.E7) EL2 South Perim Spc (G.S1) EL2 East Perim Spc (G.E2) EL2 North Perim Spc (G.N3) EL2 East Perim Spc (G.E4) EL2 North Perim Spc (G.N5) EL2 West Perim Spc (G.W6) EL2 Core Spc (G.C7) EL3 South Perim Spc (G.S1) EL3 East Perim Spc (G.E2) EL3 North Perim Spc (G.N3) EL3 East Perim Spc (G.E4) EL3 North Perim Spc (G.N5) EL3 West Perim Spc (G.W6) EL3 Core Spc (G.C7) EL4 NE Perim Spc (G.NE1) EL4 SW Perim Spc (G.SW2) Occupancy Type Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Mixed Dining Dining Notes: 1.Only spaces that are both occupied and conditioned are listed here. See Special Features section for all other spaces. 2.See LTG-1C Form. 3.Provide Tailored Lighting forms & lighting plans that demark areas with Tailored Lighting allowances. 4.Additional Tailored Allowance may......

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Dav Spc in Service Industry

...Why is DAV using SPC? Does this differ from a manufacturing company? Insurance, a mature industry, is becoming very competitive, and is more and more considered a commodity. DAV wants to keep its leading position in the insurance industry. The company, with its multi locations operations, more than 2000 people in staff, the seasonal variability of workload, the ‘same day’ processing of demands, etc… did recognize the need for consequent quality control methods. The company did note that customers subscribing to a new insurance police are very unhappy about mistakes made in their personal data and also that correcting those mistakes are time and cost intensive. DAV identified customer service as a potential vector to improve its customer satisfaction and needs a methodology to accurately and undisputedly measure the accuracy level of the registration process at New Policies’ department. Using the SPC method DAV attempts to track in a quantifiable manner how high/low the level of inaccuracy of the process (and not explicitly the employees). SPC is part of TQM and is originally designed for quality control in manufacturing environment. Manufacturing corporations do quantify the limits of a manufactured product in measurable units (variables). The challenge when transposing this method to services industries is to quantify human actions, and appreciate the limits of that action. (Attributes) ‘When is a service good or bad?‘ How would you explain to a bank manager......

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