Motility

In: Science

Submitted By cyeezy
Words 1250
Pages 5
Motility Testing
Cynthia Alonzo, M.S. Version 09-2.01
Read the entire experiment and organize time, materials, and work space before beginning.
Remember to review the safety sections and wear goggles when working with chemicals.
Objectives:
 To learn flagellar structure and arrangements common in microbes, and
 To use direct observation and testing to determine if a given microbe is motile.

Materials
From:
Label or Box/Bag: Qty Item Description:
Student
Provides 1 Microscope
1 Paper Clip 1 10%-bleach Solution 1 Paper towels
From LabPaq 1 Gloves packages - 11 pairs 1 Immersion Oil 1 Slide - Cover Glass - Cover Slip Cube (4)
Culture Media
Bag #2 -
Refrigerate
upon Receipt
Culture Media Bag #2 -
Refrigerate upon
Receipt
2 Agar, 0.4% Motility Test Agar - 8 mL in Glass
Tube
Inoculation
Instruments
Inoculation
Instruments
1 Inoculation Loop, Plastic
Mask Bag Mask Bag 1 Mask with Earloops (11) in Bag 5" x 8"
Slide Box MBK Slide Box MBK 1 Slide-Box-MBK with Blank-Slides (4)
Pre-Lab Preparation: Place saved cultures of E. coli and S. epidermidis (from previous lab) in incubator 12-24 hours prior to the start of the experiment.
Discussion and Review: Many bacteria are capable of motility, the ability to move under their own power. Most motile bacteria propel themselves by special organelles termed flagella. The bacterial flagellum is a noncontractile, semi-rigid, helical tube composed of protein and anchors to the bacterial cytoplasmic membrane and cell well by means of disk-like structures. The rotation of the inner disk causes the flagellum to act much like a propeller.
Bacterial motility constitutes unicellular behavior. In other words, motile bacteria are capable of a behavior called taxis. Taxis is a motile response to an environmental stimulus and functions to keep bacteria in an optimum environment. The arrangement…...

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